mixer mill mm300

A survey of the microbial populations in some Australian ...

The samples were milled for 1 min at 25 Hz in the Mixer Mill MM300 (Retsch, Germany). The ground coal was removed from the grinding jar and sodium dodecyl substrate was added to a final concentration of 2% and the suspension was incubated at 65 °C for 1 h.

Protocol for Total RNA Extraction/Purification – CD …

1. Freeze 2ml Eppendorf microcentrifuge tube with tissue sample and glass ball at -80°C and then grind the sample in the MM300 Mixer Mill at 30Hz for two minutes. 2. Add 1 ml fresh TRIzol Reagent to the mechanically disrupted tissue sample, vortex well, and incubate the sample at room temperature for 5 …

Prostaglandin E2 mediates acid-induced heartburn in ...

3 min) was conducted with a mixer mill (MM300; Qiagen). The protein concentration in the supernatant was measured with the BCATM protein assay kit (Thermo Fisher Scientific). The level of PGE 2 was measured by enzyme-linked immunosorbent assay (ELISA). To measure the amount of PGE 2, a Prostaglandin E 2 EIA

Used Mills Mixers for sale. Lightnin equipment & more ...

Retsch Mixer Mill. Manufacturer: Retsch; Retsch MM300 TissueLyser Lab Vibration Mill Mixer provides rapid and efficient disruption of cells and tissues for the BioRobot. Processing of up to 2 x 96 samples takes as little as 2 – 4 minutes. Disruption is ...

RETSCH MM300 Mm 300 Lab Grinder Tissuelyser Tissue …

Find many great new & used options and get the best deals for RETSCH MM300 Mm 300 Lab Grinder Tissuelyser Tissue Lyser Pulverizer Mixer Mill at the best online prices at …

Mixer Mills MM 200, MM 301

The RETSCH mixer mills MM 200 and MM 301 serve the purpose of fine and ultra-fine grinding of not only hard, medium-hard, and brittle sam-ples, but also elastic and fibrous ones. With our mills you can grind: Tissues, bones and hair Chemicals, drugs, coated and uncoated tablets Minerals, ores, and alloys Glass and ceramics

Toscana Virus RNA in Sergentomyia minuta Flies - Volume 12 ...

Five pools of the captured S. minuta were prepared with a maximum of 30 flies per pool. They were ground in 20% fetal bovine serum–enriched phosphate-buffered saline in a Mixer Mill MM300 (Qiagen, Courtaboeuf, France) with one 3-mm tungsten bead and clarified by low-speed centrifugation.

ミキサーミル MM400 | レッチェ

レッチェのミキサーミル mm400は・にしたボールミルで、にいをできます。また、オプションでマイクロチューブのホルダーをすればのにえます。

CD14 Mediates Toll-like Receptor 4 (TLR4) Endocytosis and ...

At 24 and 48 h after infection, mice were euthanized, and the whole eye was homogenized in 1 ml of PBS using the Mixer Mill MM300 (Retsch, Newtown, PA) at 33 Hz for 4 min. Serial log dilutions of the eye homogenate were plated onto brain heart infusion agar plates. After overnight incubation at 37 °C, cfu were determined by direct counting.

RETSCH MM 400 Mixer Mill MM 400 Mixer Mill:Specialty Lab ...

The mixer mill MM 400 is a compact versatile bench-top unit, which has been developed specially for dry, wet, and cryogenic grinding of small amounts of sample. The mixer mill MM 400 can mix and homogenize powders and suspensions in only a few seconds. It is also suitable for the disruption of biological cells as well as for DNA/RNA extraction.

Ocular and neural distribution of feline herpesvirus-1 ...

The Eppendorf tube was then placed in the mixer mill (MM300, Qiagen, Valencia, CA) for tissue disruption and homogenization. The resulting homogenate was frozen at –80°C. Sections of the contralateral cornea and conjunctiva and the entire contralateral TG and CG were collected for histological evaluation.

Mag-Bind® Plant DNA DS Kit

• Equipment for disrupting plant tissue (Geno/Grinder 2010 or MM300 Mixer Mill and tungsten carbide beads) • 8- or 12-channel pipette • Reagent reservoirs • Sealing film • Sealed deep-well plate or capped microtube rack for sample disruption • ethanol • isopropanol • Optional: nuclease-free water Before Starting

Retsch mm300 manually - Co-production practitioners network

Retsch mm300 manually. The Retsch MM301 Mixer Mill is used for size reduction and pulverization of hard, medium-hard and brittle samples as well as for soft, elastic or fibrous ones. They pulverize tissue, bones, hair, chemicals, drugs, coated and uncoated tablets, minerals, ores, alloys, glass, ceramics, soils, sludge, plant • Mixer mill ...

3519N MILL - Retsch MM300 grinding mill | American ...

Retsch MM300 mixer mill. 90 day warranty. The Retsch Mixer Mill MM300 is a compact versatile benchtop unit developed specifically for dry grinding of small amounts of sample. The MM300 can mix and homogenize powders very quickly, typically in just a few seconds. It is also suitable for the disruption of biological cells for DNA and RNA extraction.

A study of a population of Nyssomyia trapidoi (Diptera ...

Twelve pools, each containing 50 Ny. trapidoi sand flies (a total of 550 females and 50 males) were ground using a Mixer Mill MM300 (Qiagen) with a 3-mm tungsten bead at a frequency of 30 cycles s −1 for 3 min in the presence of 600 μl Eagle's minimal essential medium supplemented with 5% decomplemented calf serum, 1% L-Glutamine and 100 IU ...

RETSCH MM300 Mm 300 Lab Grinder Tissuelyser Tissue Lyser ...

Find many great new & used options and get the best deals for RETSCH MM300 Mm 300 Lab Grinder Tissuelyser Tissue Lyser Pulverizer Mixer Mill at the best online prices at …

Immune surveillance activation after neoadjuvant therapy ...

Briefly, 30 mg of tissue was homogenized in SV RNA Lysis Buffer using the Mixer Mill MM300 (Qiagen). RNA isolation was then performed as recommended by the manufacturer's protocol. Complementary DNA (cDNA) synthesis was performed using the Applied Biosystems cDNA Synthesis kit according to the manufacturer's directions.

Massilia Virus, A Novel Phlebovirus (Bunyaviridae ...

Detection of phleboviruses in sandfly pools by RT-PCR. Pools of entire sandflies were ground in 20% fetal bovine serum-enriched phosphate buffered saline, using a Mixer Mill MM300 (Qiagen, Courtaboeuf, France) with one 3-mm tungsten bead and 24 cycles/sec frequency for 30–60 sec, depending on the homogeneity of the material.

MK-4101, a Potent Inhibitor of the Hedgehog Pathway, Is ...

0.4 mmol/L, EDTA-free Complete, with a Mixer Mill-MM300 homogenizer (Retsch) at 30 Hz 40 00, then lysed in 1% SDS, electrophoresed, and blotted onto a nitrocellulose membrane.

Used Retsch for sale. Cyclone equipment & more | Machinio

Retsch Mixer Mill. Manufacturer: Retsch Retsch MM300 TissueLyser Lab Vibration Mill Mixer provides rapid and efficient disruption of cells and tissues for the BioRobot. Processing of up to 2 x 96 samples takes as little as 2 – 4 minutes. Disruption is ...

PBSA - Phosphate-Buffered Saline | AcronymFinder

They were ground in 20% fetal bovine serum-enriched phosphate-buffered saline in a Mixer Mill MM300 (Qiagen, Courtaboeuf, France) with one 3-mm tungsten bead and clarified by low-speed centrifugation. Toscana virus RNA in Sergentomyia minuta flies.

Total DNA isolation protocol - PrimerDigital

Mix very well for 3 minutes in the MM300 Mixer Mill at 30 Hz. Spin at maximum speed in a microcentrifuge for 2 minutes, transferred the upper aqueous layer to a new 2 ml microcentrifuge tube which contains 600 μl 2-propanol, vortex well and centrifuge the tubes at maximum speed in a microcentrifuge for 2 minutes.

Mixer Mill MM 200 - RETSCH - high performance and …

The mixer mill MM 200 is a compact versatile benchtop unit, which has been developed specially for dry grinding of small amounts of sample. It can mix and homogenize powders in only a few seconds.It is also perfectly suitable for the disruption of biological cells as well as for DNA/RNA extraction. You may also be interested in the High Energy Ball Mill Emax, an entirely new type of mill for ...

Frontiers | Foliar N Application on Tea Plant at Its ...

The dried samples were ground into a fine powder using a ball mill (Mixer Mill MM300; Retsch, Germany). To measure the total N concentration of the mature leaves, 0.1 g of each dried sample was examined in an elemental analyzer (Vario Max CN Analyzer; Elementar Analysensysteme , Germany). The concentrations of the total free amino acids and ...

Functional analysis of the HD-Zip transcription factor ...

For Southern blot analysis, rice genomic DNA was isolated from young leaves in 96 tube-tacks (Qiagen) by dry-grinding using a Mixer Mill MM300 (Retch, Germany) with 4 mm stainless steel beads followed by DNA extraction according to Pereira and Aarts .

The câ MYCâ ABCB5 axis plays a pivotal role in 5â ...

using Mixer Mill MM300 (Qiagen). After that, total RNA isolation was performed with RNeasy Mini Kit according to the manufacturer's proto-col (Qiagen). Reverse transcription was performed with the Prime-Script RT Master Mix (Perfect Real Time; TaKaRa, Shiga, Japan). The cDNA template was amplified by real-time RT-PCR using the QuantiTect

CD14MediatesToll-likeReceptor4(TLR4)Endocytosisand ...

excised and homogenized in 150 l of PBS using a Mixer Mill MM300 (Retsch). BMDMs were grown overnight and incu-batedwithantibiotic-killedPAO1for4h,andthesupernatants were used directly. Human corneal cells were grown overnight in 12-well plates (4 105 cells/well), treated with LPS, and supernatants were collected. Cytokine were determined by

Retsch MM300 grinding mill

Retsch MM300 mixer mill 90-day warranty The Retsch Mixer Mill MM300 is a compact versatile benchtop unit developed specifically for dry grinding of small amounts of sample. The MM300 can mix and homogenize powders very quickly, typically in just a few seconds. It is also suitable for the disruption of biological cells

Genomic DNA from lipid-rich tissue

• Mixer mill MM200, MM300, MM400 (Retsch®) (suitable). If other disruption devices (section 1.2) are intended to be used, consider section 2.4.2 and WARNING note in section 2.4.3! 2.4.2 Type of bead tube Bead type, disruption time, and frequency / speed must be optimized for a given sample to obtain maximal DNA yield and quality. Type of ...

Mixer Mill MM 400 - RETSCH - powerful grinding by …

The Mixer Mill MM 400 is a compact, versatile bench-top unit developed specially for dry, wet and cryogenic grinding of small sample amounts. This laboratory mill mixes and homogenizes up to 2 x 20 ml powders and suspensions within a few seconds. It is also perfectly suitable for the disruption of biological cells as well as for DNA/RNA and ...

The relationship among p53 oligomer formation, structure ...

The cells were then homogenized using a Mixer Mill MM300 (Qiagen) with glass beads for 1 min and chilled for 1 min on ice four times. In total, 25 ...

(PDF) Evaluation of a High-Throughput Protocol for ...

Macerating the ti ssue in the Mixer Mill MM300 required an additi onal 10 min . and loading the sample extracts into the ELISA plate another 3 min. On . average, the overall sap extraction time ...

Protocol for Total RNA Extraction/Purification – CD Genomics

1. Freeze 2ml Eppendorf microcentrifuge tube with tissue sample and glass ball at -80°C and then grind the sample in the MM300 Mixer Mill at 30Hz for two minutes. 2. Add 1 ml fresh TRIzol Reagent to the mechanically disrupted tissue sample, vortex well, and incubate the sample at room temperature for 5 …

Laboratory Mixer Mill MM 200 & MM 400 - RETSCH - …

A Mixer Mill will grind and homogenize small sample volumes quickly and efficiently by impact and friction. More Information at A Mixer Mi...

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